evaluation of the effect of the 47 kda protein isolated from aged garlic extract on dendritic cells

نویسندگان

hasan namdar ahmadabad department of immunology, school of medicine, tarbiat modares university, tehran, iran

mohammad hassan zuhair department of immunology, school of medicine, tarbiat modares university, tehran, iran

elahe safari department of immunology, school of medicine, tarbiat modares university, tehran, iran

mahmood bozorgmehr department of immunology, school of medicine, tarbiat modares university, tehran, iran

چکیده

objective(s)  garlic (allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. one of the major purified garlic protein components is the 47 kda protein. in this study, the effect of 47 kda protein extracted from aged garlic (age) was evaluated on mouse dendritic cell (dc) maturation in vitro. materials and methods forty seven kda protein was purified from age by ammonium sulfate precipitation and gel filtration. sds-page was used to determine the molecular weight and purity of the isolated protein. dcs were purified from spleen of balb/c mice by nycodenz centrifugation and their adhesiveness to the plastic dish. the 47 kda protein isolated from age was added to dcs medium during the overnight culture and the expression of dc surface markers was assessed via flowcytometry. results the 47 kda protein-treated dcs lowered the expression of dc maturation markers including: cd40, cd86 and mhc-ii in comparison with non-treated dcs; (median of 41% versus 47%, 84% versus 91% and 83% versus 90%, respectively) but we observed no statistical difference between the two groups. conclusion upon treatment with dcs with 47 kda protein, dcs down regulated the expression of costimulatory and mhc-ii surface molecules, which is similar to tolerogenic dc phenotype. according to the results of the present study, we found that 47 kda protein purified from age can be considered as a potential candidate to generate tolerogenic dcs in vitro.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evaluation of the Effect of the 47 kDa Protein Isolated from Aged Garlic Extract on Dendritic Cells

Objective(s)  Garlic (Allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. One of the major purified garlic protein components is the 47 kDa protein. In this study, the effect of 47 kDa protein extracted from aged garlic (AGE) was evaluated on mouse dendritic cell (DC) maturation in vitro. Mate...

متن کامل

Evaluation of the Effect of the 47 kDa Protein Isolated from Aged Garlic Extract on Dendritic Cells

OBJECTIVES Garlic (Allium sativum) is known as a potent spice and a medicine with broad therapeutic properties ranging from antibacterial to anticancer, and anticoagulant. One of the major purified garlic protein components is the 47 kDa protein. In this study, the effect of 47 kDa protein extracted from aged garlic (AGE) was evalua. MATERIALS AND METHODS Forty seven kDa protein was purified ...

متن کامل

the effect of aqueous extract of garlic on formalin-induced pain in male rats

چکیده اثر عصاره آبی سیر بر درد ناشی از آزمون فرمالین در موش صحرایی نر به کوشش نرگس اسکندری روزبهانی زمینه و هدف: گیاه سیر از خانواده لیلیاسه و گونهallium sativum بومی آسیای میانه بوده و از دوران ،باستان تاکنون به خواص درمانی متفاوت آن مثل: کاهندگی قندخون، کلسترول خون، فشار خون، اثرات مفیدآن بر دستگاه قلبی عروقی و بیماریهای انعقادی خون، اثرات آنتی اکسیداتیو، درمان بیماریهای تنفسی وگوارشی، ا...

15 صفحه اول

the effect of traffic density on the accident externality from driving the case study of tehran

در این پژوهش به بررسی اثر افزایش ترافیک بر روی تعداد تصادفات پرداخته شده است. به این منظور 30 تقاطع در شهر تهران بطور تصادفی انتخاب گردید و تعداد تصادفات ماهیانه در این تقاطعات در طول سالهای 89-90 از سازمان کنترل ترافیک شهر تهران استخراج گردید و با استفاده از مدل داده های تابلویی و نرم افزار eviews مدل خطی و درجه دوم تخمین زده شد و در نهایت این نتیجه حاصل شد که تقاطعات پر ترافیک تر تعداد تصادفا...

15 صفحه اول

the effect of taftan pozzolan on the compressive strength of concrete in the environmental conditions of oman sea (chabahar port)

cement is an essential ingredient in the concrete buildings. for production of cement considerable amount of fossil fuel and electrical energy is consumed. on the other hand for generating one tone of portland cement, nearly one ton of carbon dioxide is released. it shows that 7 percent of the total released carbon dioxide in the world relates to the cement industry. considering ecological issu...

the effect of functional/notional approach on the proficiency level of efl learners and its evaluation through functional test

in fact, this study focused on the following questions: 1. is there any difference between the effect of functional/notional approach and the structural approaches to language teaching on the proficiency test of efl learners? 2. can a rather innovative language test referred to as "functional test" ge devised so so to measure the proficiency test of efl learners, and thus be as much reliable an...

15 صفحه اول

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of basic medical sciences

جلد ۱۵، شماره ۲، صفحات ۷۴۵-۷۵۱

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023